DNA Damage and Cell Lethality by Photodynamically Produced Oxygen Radicals

BY PAULA ELLEN BURCH

A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy

RICE UNIVERSITY

HOUSTON, TEXAS

April 21, 1989

TABLE OF CONTENTS

ABSTRACT		ii
ACKNOWLEDGEMENTS		iv
LIST OF FIGURES		vii
LIST OF TABLES		xii
LIST OF ABBREVIATIONS		xiii
CHAPTER I. INTRODUCTION AND LITERATURE REVIEW		1
	Oxygen Toxicity	1
	Defenses Against Oxygen Toxicity	14
	The Photodynamic Effect	27
CHAPTER II. REACTIONS OF DYES		57
	Introduction	57
	Materials and Methods	59
	Results	61
CHAPTER III. HYDROXYL RADICAL PRODUCTION IN VITRO		82
	Introduction	82
	Materials and Methods	83
	Results	86
CHAPTER IV. DNA DAMAGE IN VITRO		116
	Introduction	116
	Materials and Methods.	117
	Results	118
CHAPTER V. DNA DAMAGE IN VIVO AND OTHER IN VIVO EXPERIMENTS		152
	Introduction	152
	Materials and Methods	158
	Results	165
CHAPTER VI. CONCLUSIONS.		178
VII. REFERENCES [have been placed at foot of each chapter]		185